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fitc conjugated se1  (Novus Biologicals)


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    Structured Review

    Novus Biologicals fitc conjugated se1
    Release of structural liver cells into the perfusate after cold ischemia. ( a ) Percentages of presumed hepatocytes (left/purple), sinusoidal endothelial cells (middle/green), and stellate cells (right/blue) in the perfusate, relative to the total number of nucleated cells (TNCs) in the perfusate. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for quantification of presumed hepatocytes <t>(CD45−/SE1−/ASGR1+/OX62−),</t> liver sinusoidal endothelial cells (LSECs) (CD45−/SE1+/ASGR1−/OX62−), and stellate cells (CD45−/CD105+/SE1−/CD14+). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 µm.
    Fitc Conjugated Se1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+conjugated+se1/Hepatic+Sinusoidal+Endothelial+Cells+Antibody+(SE-1)+%5BFITC%5D/pmc06981218-285-27-30
    Average 90 stars, based on 1 article reviews
    fitc conjugated se1 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Cell release during perfusion reflects cold ischemic injury in rat livers"

    Article Title: Cell release during perfusion reflects cold ischemic injury in rat livers

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-57589-4

    Release of structural liver cells into the perfusate after cold ischemia. ( a ) Percentages of presumed hepatocytes (left/purple), sinusoidal endothelial cells (middle/green), and stellate cells (right/blue) in the perfusate, relative to the total number of nucleated cells (TNCs) in the perfusate. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for quantification of presumed hepatocytes (CD45−/SE1−/ASGR1+/OX62−), liver sinusoidal endothelial cells (LSECs) (CD45−/SE1+/ASGR1−/OX62−), and stellate cells (CD45−/CD105+/SE1−/CD14+). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 µm.
    Figure Legend Snippet: Release of structural liver cells into the perfusate after cold ischemia. ( a ) Percentages of presumed hepatocytes (left/purple), sinusoidal endothelial cells (middle/green), and stellate cells (right/blue) in the perfusate, relative to the total number of nucleated cells (TNCs) in the perfusate. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for quantification of presumed hepatocytes (CD45−/SE1−/ASGR1+/OX62−), liver sinusoidal endothelial cells (LSECs) (CD45−/SE1+/ASGR1−/OX62−), and stellate cells (CD45−/CD105+/SE1−/CD14+). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 µm.

    Techniques Used: Imaging, Flow Cytometry, Marker, Expressing

    Alterations in liver-resident immune cell release into the perfusate after cold ischemia. ( a ) Percentage of presumed Kupffer cells (left/orange), liver-resident natural killer cells (also known as pit cells) (middle/red), and dendritic cells (right/yellow) in the perfusate, relative to the total number of nucleated cells (TNCs) that are released into the perfusate from fresh (n = 4), 24-h-cold ischemic (CI) (n = 5), and 72-h-CI (n = 4) livers. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for the quantification of presumed Kupffer cells (CD45+/CD105−/SE1−/CD14+) and pit cells (CD45+/NKRP1A+/CD3−/OX62−). Dendritic cells were manually selected from a CD45+/NKRP1A−/CD3−/OX62+ population (not shown). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression images of Kupffer cells (top), pit cells (middle), and dendritic cells (below). Scale bars: 5 µm.
    Figure Legend Snippet: Alterations in liver-resident immune cell release into the perfusate after cold ischemia. ( a ) Percentage of presumed Kupffer cells (left/orange), liver-resident natural killer cells (also known as pit cells) (middle/red), and dendritic cells (right/yellow) in the perfusate, relative to the total number of nucleated cells (TNCs) that are released into the perfusate from fresh (n = 4), 24-h-cold ischemic (CI) (n = 5), and 72-h-CI (n = 4) livers. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for the quantification of presumed Kupffer cells (CD45+/CD105−/SE1−/CD14+) and pit cells (CD45+/NKRP1A+/CD3−/OX62−). Dendritic cells were manually selected from a CD45+/NKRP1A−/CD3−/OX62+ population (not shown). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression images of Kupffer cells (top), pit cells (middle), and dendritic cells (below). Scale bars: 5 µm.

    Techniques Used: Imaging, Flow Cytometry, Marker, Expressing

    Related Articles

    Staining:

    Article Title: Cell release during perfusion reflects cold ischemic injury in rat livers
    Article Snippet: The aliquot for panel 1 was additionally stained for the detection of hepatocytes p and LSECs p with Alexa 405-conjugated SE1 (1:100; Novus Biologicals, Centennial, CO, USA; Cat# NB110-68095AF405), FITC-conjugated ASGR1 (1:50; Miltenyi Biotec, Cambridge, MA, USA; Cat# 130-109-490), and PE-conjugated OX62 (1:100; Thermo Fisher, Waltham, MA, USA; Cat# 12-1030-82) antibodies. .. The aliquot for panel 2 was additionally stained for the detection of stellate p and Kupffer p cells with Alexa 405-conjugated CD105 (1:87; Novus Biologicals; Cat# NB500-452AF405), FITC-conjugated SE1 (1:100; Novus Biologicals; Cat# NB110-68095F), and Cy3-conjugated CD14 (2:100; Bioss, Woburn, MA, USA; Cay# bs-1192R-Cy3) antibodies. .. The aliquot for panel 3 was additionally stained for the detection of pit p cells and dendritic p cells with Alexa 405-conjugated NKRP1A (1:80; Novus Biologicals; Cat# NB100-65297AF405), FITC-conjugated CD3 (1:50; Invitrogen, Carlsbad, CA, USA; Cat# 11-0030-82), and PE-conjugated OX62 (1:100; Thermo Fisher; Cat# 12-1030-82) antibodies.



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    fitc conjugated se1  (Novus Biologicals)
    90
    Novus Biologicals fitc conjugated se1
    Release of structural liver cells into the perfusate after cold ischemia. ( a ) Percentages of presumed hepatocytes (left/purple), sinusoidal endothelial cells (middle/green), and stellate cells (right/blue) in the perfusate, relative to the total number of nucleated cells (TNCs) in the perfusate. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for quantification of presumed hepatocytes <t>(CD45−/SE1−/ASGR1+/OX62−),</t> liver sinusoidal endothelial cells (LSECs) (CD45−/SE1+/ASGR1−/OX62−), and stellate cells (CD45−/CD105+/SE1−/CD14+). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 µm.
    Fitc Conjugated Se1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+conjugated+se1/Hepatic+Sinusoidal+Endothelial+Cells+Antibody+(SE-1)+%5BFITC%5D/pmc06981218-285-27-30
    Average 90 stars, based on 1 article reviews
    fitc conjugated se1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Release of structural liver cells into the perfusate after cold ischemia. ( a ) Percentages of presumed hepatocytes (left/purple), sinusoidal endothelial cells (middle/green), and stellate cells (right/blue) in the perfusate, relative to the total number of nucleated cells (TNCs) in the perfusate. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for quantification of presumed hepatocytes (CD45−/SE1−/ASGR1+/OX62−), liver sinusoidal endothelial cells (LSECs) (CD45−/SE1+/ASGR1−/OX62−), and stellate cells (CD45−/CD105+/SE1−/CD14+). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 µm.

    Journal: Scientific Reports

    Article Title: Cell release during perfusion reflects cold ischemic injury in rat livers

    doi: 10.1038/s41598-020-57589-4

    Figure Lengend Snippet: Release of structural liver cells into the perfusate after cold ischemia. ( a ) Percentages of presumed hepatocytes (left/purple), sinusoidal endothelial cells (middle/green), and stellate cells (right/blue) in the perfusate, relative to the total number of nucleated cells (TNCs) in the perfusate. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for quantification of presumed hepatocytes (CD45−/SE1−/ASGR1+/OX62−), liver sinusoidal endothelial cells (LSECs) (CD45−/SE1+/ASGR1−/OX62−), and stellate cells (CD45−/CD105+/SE1−/CD14+). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression of hepatocytes (top), LSEC (middle), and stellate cells (below). Scale bars: 5 µm.

    Article Snippet: The aliquot for panel 2 was additionally stained for the detection of stellate p and Kupffer p cells with Alexa 405-conjugated CD105 (1:87; Novus Biologicals; Cat# NB500-452AF405), FITC-conjugated SE1 (1:100; Novus Biologicals; Cat# NB110-68095F), and Cy3-conjugated CD14 (2:100; Bioss, Woburn, MA, USA; Cay# bs-1192R-Cy3) antibodies.

    Techniques: Imaging, Flow Cytometry, Marker, Expressing

    Alterations in liver-resident immune cell release into the perfusate after cold ischemia. ( a ) Percentage of presumed Kupffer cells (left/orange), liver-resident natural killer cells (also known as pit cells) (middle/red), and dendritic cells (right/yellow) in the perfusate, relative to the total number of nucleated cells (TNCs) that are released into the perfusate from fresh (n = 4), 24-h-cold ischemic (CI) (n = 5), and 72-h-CI (n = 4) livers. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for the quantification of presumed Kupffer cells (CD45+/CD105−/SE1−/CD14+) and pit cells (CD45+/NKRP1A+/CD3−/OX62−). Dendritic cells were manually selected from a CD45+/NKRP1A−/CD3−/OX62+ population (not shown). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression images of Kupffer cells (top), pit cells (middle), and dendritic cells (below). Scale bars: 5 µm.

    Journal: Scientific Reports

    Article Title: Cell release during perfusion reflects cold ischemic injury in rat livers

    doi: 10.1038/s41598-020-57589-4

    Figure Lengend Snippet: Alterations in liver-resident immune cell release into the perfusate after cold ischemia. ( a ) Percentage of presumed Kupffer cells (left/orange), liver-resident natural killer cells (also known as pit cells) (middle/red), and dendritic cells (right/yellow) in the perfusate, relative to the total number of nucleated cells (TNCs) that are released into the perfusate from fresh (n = 4), 24-h-cold ischemic (CI) (n = 5), and 72-h-CI (n = 4) livers. The perfusate recirculated during 3 hours of subnormothermic machine perfusion. Stars denote statistical significance (two-way ANOVA, followed by Tukey’s post-hoc test): *0.01 < p < 0.05; **0.001 < p < 0.01; ***0.0001 < p < 0.001; ****p < 0.0001. Error bars: SEM. ( b ) Imaging flow cytometry for the quantification of presumed Kupffer cells (CD45+/CD105−/SE1−/CD14+) and pit cells (CD45+/NKRP1A+/CD3−/OX62−). Dendritic cells were manually selected from a CD45+/NKRP1A−/CD3−/OX62+ population (not shown). Left: fresh livers. Right: 24-h-CI livers. ( c ) Representative images of surface marker expression images of Kupffer cells (top), pit cells (middle), and dendritic cells (below). Scale bars: 5 µm.

    Article Snippet: The aliquot for panel 2 was additionally stained for the detection of stellate p and Kupffer p cells with Alexa 405-conjugated CD105 (1:87; Novus Biologicals; Cat# NB500-452AF405), FITC-conjugated SE1 (1:100; Novus Biologicals; Cat# NB110-68095F), and Cy3-conjugated CD14 (2:100; Bioss, Woburn, MA, USA; Cay# bs-1192R-Cy3) antibodies.

    Techniques: Imaging, Flow Cytometry, Marker, Expressing